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关键词： Class II subdivision   Deep overbite   Maxillary distalization   Palatal miniscrew   Skeletal anchorage  

摘要： Adult Class II patients with protrusive maxillary incisors, a constricted maxillary arch, deep overbite, and reduced periodontal support are challenging to manage when orthognathic surgery is refused and preservation of the soft-tissue profile is a major concern. This case report describes the nonsurgical treatment of an adult patient with an Angle Class II division 1 right-subdivision malocclusion using maxillary third-molar extraction, self-ligating appliances, and miniscrew-assisted maxillary distalization, with successful transfer of anchorage from failed buccal miniscrews to palatal miniscrews. A 30-year-old man presented with maxillary anterior spacing, a constricted maxillary arch, excessive overjet, traumatic deep overbite, mild mandibular crowding, and generalized reduced periodontal support. Treatment was performed without premolar extraction using bite turbos, sequential archwires, intermaxillary elastics, maxillary third-molar extraction, and en masse maxillary distalization supported initially by buccal miniscrews and subsequently by palatal miniscrews after buccal screw failure. After 16 months of active treatment, facial and smile aesthetics were improved, with full maxillary incisor display and reduced buccal corridors. Bilateral Class I canine and molar relationships, coincident dental midlines, normal overjet and overbite, and good intercuspation were achieved. Periodontal re-evaluation showed a clinically stable periodontium. Successful nonsurgical correction of this adult Class II division 1 right-subdivision malocclusion was achieved without premolar extraction. Transfer of anchorage from failed buccal miniscrews to stable palatal miniscrews was a key factor in completing treatment and obtaining favourable occlusal, dentoalveolar, periodontal, and aesthetic outcomes.

关键词： NaNbO3-Based ceramics   Optical temperature sensing   Up-conversion   Non-thermally coupled levels  

摘要： Upconversion luminescence (UCL) encounters considerable obstacles in the form of thermal quenching and inadequate stability at elevated temperatures, which stem from both an increased non-radiative transition probability and host lattice defects. In this work, Na_1-3x-3yTm_xYb_yNbO₃ ceramics were evaluated for their potential in luminescence intensity ratio-based optical thermometry, utilizing the non-thermally coupled blue (¹G₄→³H₆) and red (³F₂→³H₆) emissions of Tm³⁺. The temperature-dependent UCL spectra were systematically investigated over a broad temperature range of 193-833 K. The absolute sensitivity increased gently across the temperature range of 193 - 433 K and then rose sharply after 433 K, while the relative sensitivity reached a maximum of 3.21% K⁻¹ at 353 K. As a complementary approach, the temperature-dependent average decay lifetime was evaluated for optical thermometry. Notably, this modality yielded distinct optimal temperatures for absolute sensitivity (0.17 K⁻¹ at 513 K) and relative sensitivity (0.17% K⁻¹ 673 K), highlighting the versatility of multi-parameter temperature sensing.

关键词： Coordination polymer   Copper   Dye removal   Heterogenous catalyst   Muconate  

摘要： Novel one-dimensional (1-D) coordination polymers [Cu(cc-muc)(phen)]_n (1) and [Cu(tt-muc)(phen)]_n (2), (cc-muc = cis,cis-muconate; tt-muc = trans,trans-muconate; phen = 1,10-phenantroline), were obtained by self-assembly reactions at ambient conditions. Polymers 1 and 2 were characterized by elemental analysis, IR spectroscopy, single crystal and powder X-ray diffraction and thermal analysis. 1 and 2 crystallize in monoclinic C2/c space group. In both polymers, the metal centres have a distorted octahedral coordination geometry and the coordination modes of the muc ligands, along to the chelate coordination of phen, lead to 1-D coordination arrays. These Cu(II) polymers are water stable and their efficiency as heterogenous catalysts in Fenton-like process was proved in the removal of a cationic dye, Congo red (CR), and of an anionic dye, malachite green (MG), from water. Polymers 1 and 2 can remove both organic dyes, reaching above 80% degradation of CR in approximately 30 min, under room visible light, without the need of light irradiation, and in darkness. For MG, under the same experimental conditions, over 60% degradation was obtained for both polymers after 60 min. Additionally, photoluminescence analyses in aqueous solution proved that polymer 1 is a more efficient photocatalyst than polymer 2. Furthermore, polymers 1 and 2 can perform five cycles of the photocatalytic reaction with over 75% removal of CR. PXRD analyses demonstrate that polymers 1 and 2 are structurally stable after catalyzing a complete Fenton-like CR dye removal process.

关键词： Apelin receptor   G-protein-biased agonist   Structure–activity relationship   β-Arrestin  

摘要： The apelin receptor (AplnR) is a prominent peptide-binding Class A G protein-coupled receptor (GPCR) involved in the regulation of cardiovascular, gastrointestinal, and immune functions, as well as skeletal development. Activation of the AplnR-mediated β-arrestin pathway has been associated with adverse effects including pathological cardiac hypertrophy and heart failure. Therefore, the development of G protein-biased small-molecule agonists is expected to provide an improved safety profile for long-term therapeutic use. In this study, a series of 1H-1,2,4-triazole-3-carboxamide derivatives were designed and synthesized as small-molecule AplnR agonists. Among them, the most promising compound B-007 exhibited potent agonist activity with an EC₅₀ value of 11.6 nM. Notably, compared with an endogenous ligand apelin-13, B-007 demonstrated a pronounced G protein bias with abolished β-arrestin signaling. These results identified a new promising scaffold for the development of G protein-biased agonists.

关键词： Anti-PD-1 therapy   Metastatic nasopharyngeal carcinoma   PERCIST criteria, TLG2   PET/CT  

摘要： This study investigated the prognostic value of ¹⁸F-PET/CT parameters in patients with metastatic nasopharyngeal carcinoma (NPC) treated with gemcitabine-cisplatin (GP) and programmed cell death protein 1 (PD-1) inhibitors as first-line therapy.

关键词： Dahuang Fuzi Tang   Emodin   m5C   Pyroptosis   Renal fibrosis   TRPV4  

摘要： Renal fibrosis is the hallmark pathological outcome of chronic kidney disease (CKD), and effective anti-fibrotic therapies remain scarce. This study investigates the renoprotective effects and underlying mechanisms of emodin. A rat model of renal fibrosis was established using doxorubicin hydrochloride (DXR, 4 mg/kg), and NRK-52E cells were treated with angiotensin II (Ang II, 1 μM) to induce renal fibrosis in vitro. The regulatory effects of emodin (oral gavage of 40 mg/kg/day for 4 weeks in vivo and 100 μmol/L in vitro) on renal dysfunction, fibrosis, oxidative stress, and pyroptosis were evaluated. Additionally, the role of emodin in mediating the 5-methylcytosine (m⁵C) modification of transient receptor potential vanilloid 4 (TRPV4) was investigated in vitro. The interaction between emodin and the m⁵C regulatory protein AlkB homolog 1 (ALKBH1), as well as the association between ALKBH1 and TRPV4 mRNA, were detected. Emodin significantly ameliorated renal dysfunction and attenuated fibrosis in DXR-induced rats. Emodin also restored TRPV4 expression, which was downregulated in the fibrotic models. Conversely, TRPV4 knockdown partially reversed the protective effects of emodin, leading to exacerbated activation of the transforming growth factor-β1 (TGF-β1)/Smad2/3 pathway, along with enhanced fibrosis and pyroptosis. Mechanistically, Ang II elevated global m⁵C RNA modification and upregulated the demethylase ALKBH1; these effects were reversed by emodin. Furthermore, ALKBH1 overexpression promoted the destabilization of TRPV4 mRNA via site-specific m⁵C demethylation. Emodin alleviates renal fibrosis by preserving TRPV4 expression through targeting the ALKBH1-dependent m⁵C demethylation axis. Targeting the ALKBH1–m⁵C–TRPV4 axis may represent a promising therapeutic strategy for renal fibrosis.

关键词： Cold-active   GH64   Glucanase   Laminaripentaose   Structural analysis  

摘要： β-1,3-Glucans are ubiquitous polysaccharides whose enzymatic hydrolysis yields β-1,3-gluco-oligosaccharides (GOS) with immunomodulatory, antioxidant, and prebiotic properties. These bioactivities highlight their potential as functional food ingredients. However, conventional enzymatic methods often yield structurally heterogeneous GOS, complicating their application. Glycoside hydrolase family 64 (GH64) enzymes are unique in producing laminaripentaose as a single dominant product, enabling the preparation of structurally uniform GOS. Here, a novel GH64 enzyme, CsGH64, from Candidatus saccharibacteria was biochemically and structurally characterized. CsGH64 exhibited strict β-1,3-glucanase activity, producing laminaripentaose at > 80% yield, with optimal activity at pH 6.0 and 20 °C. Kinetic analysis revealed a Km of 27.9 mg mL⁻¹ and a Vmax of 16.3 U mg⁻¹. Comparative structural analysis with previously characterized GH64 enzymes uncovered distinct features underlying its cold-adapted catalytic properties. These results identify CsGH64 as a promising biocatalyst for the food and nutraceutical industries, enabling the efficient and mild production of structurally defined, bioactive oligosaccharides.

关键词： Complex   Crystal structure   Fluorescence sensor  

摘要： In this paper, 3, 5-bis-(triazol-1-yl)-benzoic acid (HL1) and 2, 4-bis-(triazol-1-yl)-benzoic acid (HL2) are used as ligands to obtain two complexes of [Zn(L1)₂]∙0.25H₂O (1) and [Zn₂(L2)₄] (2). Single crystal X-ray diffraction analysis shows that complex 1 forms a three-dimensional structure similar to the interlocking zipper, and complex 2 exhibits an occlusal two-dimensional framework with cavities. The solid-state fluorescence properties of complexes 1–2 were further investigated to explore their applications. The results show that the fluorescence of these two complexes are very different, in which 1 displays very impressive fluorescence compared with 2. Thus, complex 1 was further studied as a chemical sensor and it is interesting to find that this complex can be used to detect Cr₂O₇²⁻ (LOD: 1.29 μM) by fluorescence quenching and DMSO by fluorescence enhancement. At the same time, complex 1 also has fluorescence enhancement effect in the related cation sensing experiments. On the basis of these experiments, the fluorescence quenching mechanism was also screened, and the energy transfer between the analyte and the complex is the main point of fluorescence quenching. In addition, to explore the extendable properties of the complexes, the dye adsorption experiments were also carried out. Interestingly, complex 2 displays certain adsorption capacity for different types of organic dyes of CR, MG, CV, MB, FY and OG within 30 min.