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关键词： inositol   MYO:DCI ratio   polycystic ovary syndrome (PCOS)   antioxidants   In vitro fertilization (IVF)  

摘要： Background PCOS is frequently associated with metabolic disturbances, including a high prevalence of insulin resistance and compensatory hyperinsulinemia, which can impair reproductive outcomes. Previous evidence indicates that combining myo-inositol and D-chiro-inositol in a specific 3.6:1 ratio may support ovarian physiology and reproductive performance in women with PCOS. This study aimed to evaluate whether taking a 3.6:1 MYO:DCI inositol-based supplement enriched with antioxidants for at least 1 month prior to controlled ovarian stimulation could improve IVF-ICSI outcomes. Methods A retrospective observational cohort study was conducted, including 92 women diagnosed with PCOS according to the Rotterdam criteria. Patients were allocated to 2 groups: those who used the MYO:DCI 3.6:1 antioxidant-containing supplement (Group 1) and those who followed conventional supplementation (Group 2). Outcomes assessed included ovarian response, fertilization rates, blastocyst development and pregnancy results. Results The number of retrieved oocytes (21.5 in Group 1 vs. 20.7 in Group 2;p = 0.56) and the metaphase II oocyte rate (72.06% in Group 1 vs. 71.23% in Group 2;p = 0.68) were comparable between both groups. Notably, Group 1 achieved higher fertilization rates (77.66% vs. 71.61%;p = 0.0314) and a lower proportion of cycles without at least one good-quality blastocyst to transfer (4.35% vs. 17.39%;p = 0.0450). In addition, both clinical and ongoing pregnancy rates were significantly improved in the supplemented group (54.39% vs. 42.65%;p = 0.0203;45.61% vs. 36.76%;p = 0.0033). Conclusion This observational pilot study suggests that a pre-treatment with 3.6:1 MYO:DCI ratio and antioxidant-based supplement taken for at least 1 month prior to ovarian stimulation may improve oocyte quality and increase pregnancy rates. These findings, while promising, require confirmation in larger prospective trials.

关键词： Winter wheat mapping   multi-source and multi-temporal   feature selection   Jeffreys-Matusita distance   VGG16-UNet++ hybrid network  

摘要： Accurate winter wheat mapping from satellite data is essential for food-security assessment and regional agricultural management, but is hampered by single-source imagery, feature redundancy and limited generalization. We propose a compact deep-learning framework that fuses multi-temporal Sentinel-1/2 data with JM-guided feature selection for county-scale winter wheat mapping. In Taikang County, Henan Province, a phenology-aware 124-dimensional feature set integrating spectral, index, texture and polarization descriptors is constructed, and the Jeffreys-Matusita distance is used to retain 46 discriminative features, reducing dimensionality by 62.9% while preserving class separability. Three segmentation networks-VGG16, UNet++ and a lightweight hybrid VGG16-UNet++-are evaluated;the hybrid model with JM-selected features performs best, achieving PA 90.53%, UA 90.90%, OA 96.99%, Kappa 0.9525, IoU 0.7883 and F1 0.9071 with moderate complexity. When directly transferred to neighbouring Biyang County without retraining, the hybrid network still yields accurate and spatially coherent winter wheat maps, providing initial evidence of cross-county transferability for regional monitoring.

关键词： Contrast-induced acute kidney injury   roxadustat   ROS   Nrf2/HO-1   inflammation   oxidative stress  

摘要： Roxadustat (ROX) has a protective action against contrast-induced acute kidney injury (CI-AKI). Excessive production of reactive oxygen species (ROS) plays a significant role in exacerbating the deterioration of renal function. Notably, activation of Nrf2/HO-1 signaling can reduce ROS production. We constructed a mouse model of CI-AKI by iohexol injection. The effect of ROX on CI-AKI was assessed by measuring serum blood urea nitrogen (BUN) and creatinine (Cr evaluating renal histopathology, and analyzing the expression level of Nrf2 and HO-1. Additionally, levels of ROS, malondialdehyde (MDA proinflammatory cytokines (IL-6, TNF-alpha, and IL-1 beta as well as the activities of antioxidant kinases superoxide dismutase (SOD) and glutathione (GSH were examined. The results showed that ROX significantly suppresses the iohexol-induced decline in the activity of antioxidant enzymes, increases the levels of ROS, MDA, Cr, IL-6, IL-1 beta, BUN and TNF-alpha, and attenuates renal pathological injury. Furthermore, ROX enhanced the iohexol-induced increase in Nrf2 and HO-1 expression. However, these effects of ROX on CI-AKI were abolished by ML-385, a Nrf2 inhibitor. Our findings indicate ROX attenuates CI-AKI by down-regulating inflammation and restoring the balance between oxidative stress and anti-oxidant defenses. This may involve the activation of the Nrf2/HO-1 signaling pathway to clear excessive ROS.

关键词： Soluble E-selectin   soluble vascular cell adhesion molecule-1   neonatal respiratory distress syndrome   diagnosis   prognosis   logistic regression  

摘要： Objective: This study evaluates the clinical significance of soluble E-selectin (sE-selectin) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in umbilical cord blood for neonatal respiratory distress syndrome (NRDS). Methods: This retrospective single-center study collected 558 preterm infants, categorized into NRDS (n = 162) and non-NRDS (n = 396) groups. sE-selectin and SVCAM-1 levels in umbilical cord were measuredcorrelations with the 5-minute Apgar score and PaO/FIO, ratio were assessed using Spearman/Pearson analysis. ROC curves were generated to assess their predictive value for NRDS diagnosis and discriminatory value for 28-day prognosis. Logistic and Poisson regression models were used to identify risk factors for poor prognosis. Results: sE-selectin and SVCAM-1 were elevated in NRDS infants and positively correlated with disease severity. They showed negative correlations with 5-minute Apgar score and PaO/FiO, ratio. For NRDS prediction, the AUC was 0.894 for sE-selectin and 0.878 for SVCAM-1. sE-selectin combined with SVCAM-1 achieved an AUC of 0.935. For discriminating poor 28-day prognosis, the AUC was 0.867 for SE-selectin and 0.878 for SVCAM-1. The combined AUC of 0.913 was higher than that of any biomarker alone. SE-selectin (OR: 1.163, 95%CI: 1.057 similar to 1.279 SVCAM-1 (OR: 1.097, 95%CI: 1.035 similar to 1.163 and 5-minute Apgar score (OR: 0.408, 95%CI: 0.269 similar to 0.620) were independently associated with poor prognosis. Poisson regression analysis results in the generalized linear model were consistent with the conclusions of the logistic regression analysis. Conclusion: SE-selectin and SVCAM-1 are elevated in umbilical cord blood in NRDS infants and closely correlated with disease severity and 28-day prognosis.

关键词： Interleukin-1 beta   osteogenic differentiation   periodontal ligament stem cells   periodontitis   TRAB domain-containing protein 2A   tumor necrosis factor-alpha  

摘要： Background Periodontitis is a chronic inflammatory condition characterized by destruction of periodontal support tissues and dysregulated immune responses, particularly involving cytokines like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta). Identifying molecules that regulate both inflammation and tissue regeneration is essential for targeted therapies. Following preliminary transcriptomic evidence on periodontal tissues, this study aimed to define the specific immunomodulatory and osteogenic regulatory effects of TRAB domain-containing protein 2A (TRABD2A).Methods RNA sequencing was performed on periodontal tissues from patients with periodontitis and periodontally healthy individuals to identify differentially expressed genes and conduct functional enrichment analysis. TRABD2A expression and its effects on cell proliferation, inflammation, osteogenic markers expression, and mineralization were assessed in human periodontal ligament stem cells (hPDLSCs) treated with recombinant TRABD2A, Porphyromonas gingivalis lipopolysaccharide (LPS or a TRABD2A inhibitor (o-Phenanthroline).Results RNA sequencing revealed significant TRABD2A upregulation in periodontitis tissues, along with elevated bone-related genes runt-related transcription factor 2 (Runx2) and receptor activator of nuclear factor-kappa B Ligand (RANKL) expression. In vitro experiments demonstrated that TRABD2A reduced the secretion of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) and promoted the proliferation of hPDLSCs. Furthermore, TRABD2A upregulated Runx2 expression and suppressed RANKL expression, while Alizarin Red staining results further confirmed its role in promoting osteogenic differentiation. The underlying mechanism may be associated with the enhancement of beta-catenin expression.Conclusion TRABD2A exerts dual functions by promoting osteogenic differentiation and modulating inflammatory responses, highlighting its potential as an immuno

关键词： Double-filtration plasmapheresis   hyperviscosity syndrome   nafamostat mesylate   hypotension   technical complication  

摘要： Double-filtration plasmapheresis (DFPP) is increasingly used for immune-mediated and hematologic diseases, yet real-world safety data remain limited. In this 15-year retrospective study, we analyzed 1,022 DFPP sessions performed in 385 patients. The primary outcomes were hypotension and technical complications. Patient demographics, indications, laboratory parameters, treatment prescriptions, anticoagulation regimens, and procedure-related adverse events were collected. Mixed-effects logistic regression models were applied to identify factors associated with hypotension and technical complications. Hyperviscosity syndrome was the most common indication, accounting for 55.3% of all sessions. The mean treatment volume was 3.06 +/- 0.41 L, corresponding to 0.95 +/- 0.16 times the estimated plasma volume. Hypotension occurred in 15.3% of sessions and technical complications in 7.6%, both demonstrating significant declining trends over time (p < 0.05 for trend). Immune thrombotic thrombocytopenic purpura was independently associated with hypotension (OR 11.96, 95% CI 1.94-73.68). Older age modestly increased risk (OR 1.03 per year, 95% CI 1.00-1.06 while treatment year was inversely associated with hypotension (OR 0.77 per year, 95% CI 0.66-0.89). Technical complications were independently associated with use of the PLASAUTO Sigma system (OR 8.78, 95% CI 2.76-27.97 use of non-heparin/non-nafamostat anticoagulants (OR 9.42, 95% CI 2.71-32.72 and hyperviscosity syndrome (OR 8.09, 95% CI 1.80-36.29). Among 309 sessions with paired measurements, median immunoglobulin reduction was 29.8% for IgG overall. DFPP demonstrated an acceptable safety profile, with declining complication rates over time. The risk of complications was influenced by underlying disease, patients' status, and anticoagulation strategy.

关键词： CTRP1   hypertension   left ventricular hypertrophy   adipocytokines   biomarker  

摘要： Background: Left ventricular hypertrophy (LVH) is a critical complication of hypertension that correlates with increased morbimortality. Its pathophysiology is complex and multifaceted likely involving various players that are still to be determined, particularly those with proinflammatory and profibrotic effects. Complement C1q/tumour necrosis factor-related protein 1 (CTRP1) is an antihypotensive adipokine that has recently been linked to adverse cardiometabolic changes and may contribute to the development of LVH. Objective: To explore the relationship between CTRP1 and LVH in patients with essential hypertension. Methods: A total of 360 patients with mild-to-moderate essential hypertension were enrolled from Ruijin Hospital between December 2015 and November 2017. Participants were divided into two groups: those with hypertension alone (n = 183) and those with hypertension complicated by LVH (n = 177). Plasma levels of CTRP1, adiponectin, and interleukin-6 (IL-6) were measured using enzyme-linked immunosorbent assay (ELISA). The left ventricular mass index (LVMI) was calculated from echocardiographic measurements. Patients were further stratified by sex and by CTRP1 tertiles for subgroup analysis. Results: Patients with hypertension and LVH showed significantly higher levels of CTRP1, IL-6, and LVMI compared to those with hypertension alone. In contrast, adiponectin levels were significantly lower in the LVH group. CTRP1 levels were positively correlated with LVMI in both males and females. Furthermore, patients in the highest CTRP1 tertile exhibited progressively elevated SBP, DBP, CRP, IL-6, and LVMI. Multivariate logistic regression analysis identified CTRP1, IL-6, and adiponectin as independent factors associated with LVH. Conclusion: CTRP1 is independently associated with left ventricular hypertrophy in patients with essential hypertension, demonstrating a dose-response relationship with cardiac hypertrophy and inflammatory markers.

关键词： Parkinson's disease   N-(prop-2-yn-1-yl)-1,2,3,4-tetrahydronaphthalen-1-amine derivatives   monoamine oxidase B   neuroprotective agents   selective inhibitors  

摘要： Monoamine oxidase B (MAO-B) inhibitors may be an effective therapeutic approach for Parkinson's disease. This study designed and synthesised a series of N-(prop-2-yn-1-yl)-1,2,3,4-tetrahydronaphthalen-1-amine derivatives and evaluated their inhibitory activity against human MAO-B (hMAO-B). Most compounds exhibited inhibitory activity and selectivity, with compounds 29 and 34 demonstrating the strongest inhibitory potency (IC50 = 0.066 +/- 0.03 mu M and 0.070 +/- 0.002 mu M, respectively) and selectivity indices (SI > 151 and 134 which were superior to the positive control rasagiline. Enzyme kinetic studies confirmed that these representative active compounds exhibited mixed reversible inhibition of hMAO-B. Molecular docking and kinetic analyses indicated that compound 29 binds stably to the hMAO-B active site. Concurrently, they exhibited low neurotoxicity and protective effects against 6-OHDA-induced damage in SH-SY5Y neuroblastoma cells. Therefore, we propose these active compounds as potential drug candidates for further investigation.

关键词： Child health   community health   physical activity   nutrition   storytelling  

摘要： PurposeStorytelling has long conveyed knowledge, wisdom, and values across generations, making it a powerful tool for shifting perspectives and encouraging behaviour change. By engaging audiences emotionally, it creates a shared space where diverse viewpoints coexist, fostering empathy and understanding. This is especially valuable in public health, where translating scientific evidence into relatable, actionable knowledge is essential. The First 1,000 Days storytelling initiative in Burnie, Northwest (NW) Tasmania, demonstrated the benefits of community-driven narratives in improving the health and well-being of children from conception to age two. This study explored how storytelling could function as a core strategy within the broader initiative, while examining parents' lived experiences in workshops designed to empower healthier lifestyles.MethodsEarly years practitioners and parents collaborated around four pillars-connection, nutrition, caring, and moving. Participants shared and reshaped personal narratives linked to each area. Facilitators emphasized dignity, autonomy, and inclusivity, creating a safe environment, particularly for disadvantaged participants.ResultsParticipants combined expert knowledge with lived experience, building capacity to promote early childhood development across their networks and communities.ConclusionThis initiative illustrates how co-created storytelling effectively bridges lived experience and professional expertise, fostering reflection, empowerment, and sustainable, community-driven change in early childhood and maternal health.

关键词： cPKC gamma   spermatogonia   mitophagy   UCHL1/HIF-1 alpha   apoptosis  

摘要： Cryptorchidism, among the most frequent congenital urogenital anomalies in male infants, has a global prevalence ranging from 1% to 9%. Characterized by failure of testicular descent into the scrotum, such a condition exposes the testes to elevated temperature, oxidative stress, and impaired spermatogenesis, significantly increasing risks of infertility and testicular cancer. Although orchiopexy is the standard treatment, long-term spermatogenic defects often persist, highlighting the need to elucidate underlying molecular mechanisms. Although the main mechanistic experiments were performed in GC-1 spg cells through genetic manipulation of cPKC gamma, an additional heat-stress experiment was included to simulate the high-temperature condition associated with cryptorchidism. In the present study, the role of conventional protein kinase C gamma (cPKC gamma) in regulating mitophagy and apoptosis was investigated using mouse spermatogonial GC-1 cells. Through siRNA-mediated knockdown and adenovirus-induced overexpression, cPKC gamma upregulation was demonstrated to significantly induce cellular apoptosis and disrupt mitochondrial function, indicated by elevated malondialdehyde (MDA) levels, reduced superoxide dismutase (SOD) activity, decreased ATP production, increased 8-hydroxy-2 '-deoxyguanosine (8-OHdG) accumulation, increased mitochondrial reactive oxygen species (ROS and loss of mitochondrial membrane potential. Mechanistically, cPKC gamma was found to inhibit the UCHL1/HIF-1 alpha signaling pathway, suppressing mitophagy, as evidenced by a reduced LC3-II/LC3-I ratio and decreased PINK1 and Parkin expression. Importantly, adverse effects induced by cPKC gamma overexpression were substantially rescued through UCHL1 or HIF-1 alpha overexpression, or rapamycin treatment. Conversely, knockdown of UCHL1 or HIF-1 alpha partially counteracted the protective effects of cPKC gamma silencing on cell viability, oxidative stress, and mitophagy activation. Furthermore, heat st