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关键词： Biomanufacturing   Cell-free catalytic system   Enzyme compatibility   1,6-Hexanediamine   α,ω-diamines  

摘要： Biomanufacturing, with its advantages of mildness, low carbon emissions, and renewability, drives the frontier of green chemistry and sustainable development. However, complex metabolic pathways and intracellular resource competition frequently limit the efficiency and yield of metabolic engineering approaches, particularly for non-natural compounds such as 1,6-hexanediamine (1,6-HMD), an important precursor for nylon 66. To address these challenges, we developed a cell-free biocatalytic system employing 6-aminocaproic acid (6-ACA), derived from low-cost caprolactam, together with optimized enzyme assembly and cofactor regeneration. As a result, the 1,6-hexanediamine titer was enhanced by 511-fold, reaching a record-high level of 10.35 g/L with a conversion rate of 97.4%. The system was further extended to the production of C7–C10 α,ω-diamines, achieving high-level titers. In addition, the cell-free biocatalytic system retained considerable activity after 7 days of storage at 4 °C, with a 25.9% reduction in titer. This cell-free biocatalysis overcame the inherent limitations of biological systems and preserved enzyme activity, demonstrating the substantial potential for efficient diamine synthesis and indicating broad applicability for future practical implementations.

摘要： Correction: BMC Cancer 25, 613 (2025) Following publication of the original article [1], it has been decided to update the Competing interest to reflect the below changes. Remove “The remaining authors declare that they have no competing interests.” Add “DT is a co-founder, stakeholder, and Chief Medical Officer (CMO) of SILON Therapeutics.” The original article has been updated.

摘要： In this article [1], the graphics relating to Figs. 2 and 3 captions had been interchanged;the figure(s) should have appeared as shown below. The original article has been corrected. Incorrect Figures 3D-CT images of UPJO in six patients with HSK. The green arrows indicate the area where the crossing vessels cross the UPJ A representative 3D-CT image and an intraoperative finding in Patient 6 Correct Figures 3D-CT images of UPJO in six patients with HSK. The green arrows indicate the area where the crossing vessels cross the UPJ A representative 3D-CT image and an intraoperative finding in Patient 6

摘要： Correction: Lipids in Health and Disease 24, 153 (2025) Following publication of the original article [1], the authors reported an error in the ordering of Figs. 2 and 3. Specifically, the figures appear to be switched. Incorrect Fig. 2: Correct Fig. 2: Incorrect Fig. 3: Correct Fig. 3: The original article [1] has been corrected.

摘要： Following the publication of the original article [1], the authors proposed a correction. The response categories in the questionnaire in 2005 did not include a ‘Don’t know’ option, whereas this option was present in the questionnaires in both 2000 and 2023. The syntax used to dichotomize responses in the screening instrument was by mistake consistently based on the 2005 response categories across all three survey waves. There is no change in the results or conclusions. The changes are as follows: In the RiBED‑8 subsection, the first sentence should be revised by adding ‘(excluding ‘Don’t know’)’ at the end of the sentence before ‘(Table 1)’. The correct sentence should read: Self-reported information on ED risk behaviours was obtained by the validated screening instrument, RiBED 8, which is an interval scale that includes eight items with four response categories each ‘(excluding ‘Don’t know’) (Table 1) [31]. Table 1 needs to be corrected. The incorrect Table 1 is a follows: The eight items in the RiBED-8 screening instrument Item Confirmatory response categories Negative response categories I have a bad conscience when I eat sweets Very often, often Rarely, never, don’t know I am on a diet Very often, often Rarely, never, don’t know I am content with my eating habits Rarely, never Very often, often, don’t know I throw up to get rid of what I have eaten Very often, often, rarely Never, don’t know I feel uncomfortable when I eat with others Very often, often Rarely, never, don’t know I am afraid I cannot stop eating if I first get started Very often, often Rarely, never, don’t know I feel that it is necessary to keep a strict diet or to stick to other diet rituals to control what I eat Strongly agree, agree Disagree, strongly disagree, don’t know I feel that my desire to lose weight is out of control Strongly agree, agree Disagree, strongly disagree, don’t know The correct Table 1 is as follows: The eight items in the RiBED-8 screening instrument Item Confirmatory r

摘要： Following the publication of the original article [1], we were notified that the first author’s last name needs to be changed from Horn to Schäfer. The original article has been corrected.

关键词： Biomarker   Mastitis   Sheep   Somatic cell count   Testican-1  

摘要： The aim of this study was to evaluate serum Testican-1 levels in the diagnosis of clinical and subclinical mastitis in sheep. The animal material of the study consisted of a total of 60 Awassi sheep. The sheep were divided into three groups according to clinical examination findings, somatic cell count (SCC), California Mastitis Test (CMT) results, and bacteriological isolation and identification findings: group 1 (n = 20) consisted of healthy sheep, group 2 (n = 20) consisted of sheep with subclinical mastitis, and group 3 (n = 20) consisted of sheep with clinical mastitis. Blood samples were taken from the jugular vein and centrifuged to obtain serum. Serum C-reactive protein (CRP), serum amyloid A (SAA), and Testican-1 levels were determined in the serum samples using commercial ELISA kits. The obtained data were evaluated using one-way analysis of variance (ANOVA). Statistically significant differences were found between the groups in terms of body temperature, heart rate, respiratory rate, white blood cell (WBC) count, somatic cell count, CRP, SAA, and Testican-1 levels (P < 0.001). In the mastitis groups (clinical and subclinical), all of these parameters were found to be higher than in the control group (P < 0.001). In addition, significant and strong positive correlations were found between Testican-1 levels and body temperature, heart rate, respiratory rate, WBC, CRP, SAA, and somatic cell count (P < 0.01). In conclusion, these findings suggest that Testican-1 can be used as a potential biomarker in the diagnosis of mastitis in sheep.

摘要： The originally published version of this article contained mistakes originated with the editorial team, and the authors would like to correct it. In the published article, The "Note:" that should be part of the figure captions were incorrectly placed as part of the body texts. The original article has been corrected.

关键词： Early skin-to-skin contact   Mother-to-infant bonding   Mother-to-Infant Bonding Scale  

摘要： Background: To prevent bonding disorder, support should be provided both immediately after childbirth and in the long term. This study focuses on the prevention of bonding disorder and aimed to clarify the effect of early mother–infant contact on mother-to-infant bonding 1 year after childbirth. Methods: The participants were 81,634 mothers involved in an ongoing nationwide birth cohort study called the Japan Environment and Children’s Study. Mother-to-infant bonding was measured using the Mother-to-Infant Bonding Scale, Japanese version (MIBS-J). Mother–infant contact after childbirth was classified as “no contact”, “clothed or swaddled contact”, and “skin-to-skin contact”. Mother–infant bonding disorder was identified based on an MIBS-J score of ≥ 5, and analyses were also performed based on the sub-scores for “Lack of Affection” and “Anger and Rejection”. A generalized linear mixed model analysis was used to derive adjusted odds ratios (AORs) and 95% confidence intervals (CIs) for cases of bonding disorder and subscale scores according to mother–infant contact, with no contact as the reference group and while controlling for potential confounders. Results: One year after childbirth, the clothed or swaddled contact group (AOR 0.93, 95% CI 0.87–0.99) and the skin-to-skin contact group (AOR 0.85, 95% CI 0.81–0.90) had a significantly lower odds of bonding disorder compared with the no contact group. By subscale, the odds of bonding disorder on the Lack of Affection subscale was lower in the skin-to-skin contact group (AOR 0.91, 95% CI 0.89–0.93) than in the no contact group. For the Anger and Rejection subscale, the odds of bonding disorder was lower in the clothed or swaddled contact group (AOR 0.94, 95% CI 0.92–0.96) and in the skin-to-skin contact group (AOR 0.92, 95% CI 0.91–0.94) than in the no contact group. Conclusions: Early skin-to-skin contact between mother and child immediately after childbirth is associated with a reduced likelihood of long-term bonding

摘要： The Editor-in-Chief has retracted this article. After publication, it was found that in Fig. 3, the panel for adipogenic differentiation (in ADSCs) appeared to overlap with Fig. 3, BMSCs panel B (adipogenic differentiation), with rotation, of a previous article by the same author group [1]. The authors submitted a replacement panel which could not be validated to the time of the original experiment, and so could not be used to correct the article. The Editor has lost confidence in the data and conclusions of this article. Nagy Abouquerin, Samah H. El-Bably, Asmaa M. Ibrahium,, Mona M. Khaled, Ahmed Ismael Abdelgalil and Aya M. Yassin agree with this retraction. Mohamed A. El-Saied and Hamdy Rizk did not respond to correspondence from the publisher regarding this retraction.